murine il 22  (MedChemExpress)

Journal: Nature Communications

Article Title: Sialic acid exacerbates polycystic ovary syndrome in mice by modulating gut microbiota-mediated bile acid metabolism and FXR activation

doi: 10.1038/s41467-026-71365-4

Figure Lengend Snippet: A , B Ileal mRNA levels of Il−22 and Reg3β ( A ), and serum IL-22 levels ( B ) in Neu5Ac-treated mice ( n = 5 mice per group). C , D Ileal mRNA levels of Il−22 and Reg3β ( C ), and serum IL-22 levels ( D ) in L. sa -treated mice ( n = 5 mice per group). E – G Ileal mRNA levels of Il−22 and Reg3β , and serum IL-22 levels in CAPE- ( E ) and Gly-β-MCA-treated mice ( F ), and TUDCA-treated Fxr △IE mice ( G , n = 5 mice per group). H Schematic diagram illustrating the experimental design of rMuIL-22 treatment. I GTT and ITT assays ( n = 5 mice per group). GTT: ## p = 0.0046 (15 min), # p = 0.0136 (30 min), ## p = 0.0083 (60 min) and # p = 0.0372 (90 min) for rMuIL-22 + Neu5Ac + DHEA vs Neu5Ac + DHEA. ITT: ## p = 0.0044 (30 min) for rMuIL-22 + Neu5Ac + DHEA vs Neu5Ac + DHEA. J Estrous cycle assessment based on vaginal cytology. K Representative H&E-stained images (scale bar: 200 μm). L Quantification of cystic follicles and corpora lutea ( n = 5 mice per group). Data are presented as mean ± SD. One-way ANOVA followed by Tukey’s post hoc test ( A – G , J and L ), and two-way repeated-measures ANOVA with Sidak’s multiple-comparisons correction ( I ) were performed.

Article Snippet: For IL-22 treatment, mice were injected with recombinant murine IL-22 (rMuIL-22, HY-P7079, MCE) at 1 mg/kg daily for 21 days .

Techniques: Staining

Journal: Journal of Translational Medicine

Article Title: Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancement

doi: 10.1186/s12967-026-07757-3

Figure Lengend Snippet: Low-dose 5-HMF sensitizes intestinal crypts to IL-22‒STAT3 signaling and enhances organoid growth. ( A , B ) organoid formation assay. Crypts isolated from control or low-dose 5-HMF-treated mice were cultured with recombinant murine IL-22 (rmIL22; 50 ng/mL) for 3 days. Representative time-lapse images ( A ) and quantification of the organoid surface area (μm 2 ) from day 1 to day 3 after cultivation ( B ) are shown. ( C ) comparison of organoid growth stimulated by rmIL22 and CHIR99021 (3 μM), a Wnt/β-catenin agonist. Representative images of MTT-stained organoids. ( D ) MTT viability analysis (% positive, n = 6 wells/group). ( E , F ) immunoblot analysis ( E ) and quantification ( F ) of STAT3 phosphorylation (Tyr705) in organoids treated with or without rmIL22 (50 ng/mL, 48 h). The data are presented as the mean±SD. Statistical significance compared with the regular diet group. * p < 0.05, ** p < 0.01 and NS, no significant difference

Article Snippet: Recombinant murine IL-22 protein (rmIL22) and CHIR99021 were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Tube Formation Assay, Isolation, Control, Cell Culture, Recombinant, Comparison, Staining, Western Blot, Phospho-proteomics

Journal: Journal of Translational Medicine

Article Title: Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancement

doi: 10.1186/s12967-026-07757-3

Figure Lengend Snippet: 5-HMF upregulates IL-22 receptor expression through HIF-dependent transcriptional activation. ( A , B ) expression of IL-22 receptor subunits in intestinal crypts. ( A ) mRNA and ( B ) protein levels of IL22R1 and IL10R2 in mice treated with vehicle, low-dose (50 mg/kg), or high-dose (100 mg/kg) 5-HMF. ( C ) Bioinformatics analysis of the IL22R1 promoter region. The schematic illustrates the positions of four putative hypoxia response elements (HREs; consensus core sequence) within the murine IL22R1 locus. Supporting evidence from publicly available human ChIP-seq data (GEO database) is shown below. The track labeled “Batch4_CHROM1_LOVO_EPAS1_RABBIT” visualizes HIF-2α (encoded by EPAS1) binding sites on chromosome 1 in the LOVO human colon epithelial cell line (experimental batch 4; rabbit antibody). The prominent peak (red box) indicates significant enrichment of HIF-2α binding within the upstream regulatory region of the human IL22R1 gene, supporting its status as a direct transcriptional target under HIF-mediated regulation. ( D ) mouse IL22R1 promoter scheme showing distal (−1525~-1449 bp) and proximal (+36 ~ +74 bp) HERs identified by sequence analysis. ( E ) dual-luciferase reporter assay in HEK-293 cells with an IL22R1 promoter-luciferase construct containing a wild-type sequence (−2000 wt) or mutations of distal or/and proximal HERs with transactivation by HIF-1, HIF-2, or GFP-control plasmids ( n = 3 transfections/group). Asterisks (*) indicate the comparison of HIF1 or HIF2 to their respective GFP controls. ** p < 0.01, * p < 0.001 and NS, no significant difference. The cross (†) indicates a comparison of the transactivation of the wild-type sequence to the sequences mutated by HIF1 (†) or by HIF2 (†). † p < 0.05. All the error bars represent the means±sds

Article Snippet: Recombinant murine IL-22 protein (rmIL22) and CHIR99021 were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Activation Assay, Sequencing, ChIP-sequencing, Labeling, Binding Assay, Luciferase, Reporter Assay, Construct, Control, Transfection, Comparison

Journal: Journal of Translational Medicine

Article Title: Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancement

doi: 10.1186/s12967-026-07757-3

Figure Lengend Snippet: HIF-2α is required for 5-HMF-induced IL22R1 expression and the functional potentiation of IL-22 signaling. ( A , B ) HIF-2α inhibition abolishes 5-HMF-induced upregulation of IL22R1 at the protein level in intestinal crypts from mice treated with low-dose 5-HMF with or without the HIF-2α inhibitor PT-2385. ( C ) MTT-stained organoids were isolated from the mice in this study and cultured in medium in the presence or absence of rmIL22 and/or PT-2385 ( n = 3, 3 replicates/group). ( D ) quantification of the organoid surface area (μm 2 ×10 3 ) and MTT viability analysis (% positive, n = 6 wells/group). Statistical significance compared with the control group. * p < 0.05, ** p < 0.01, * p < 0.001 and NS, no significant difference. ( E ) schematic model summarizing the proposed mechanism by which low-dose 5-HMF mitigates radiation-induced intestinal injury. 5-HMF inhibits prolyl hydroxylase domain (PHD) enzyme activity, leading to the selective stabilization of HIF-2α in intestinal crypt cells. HIF-2α translocates to the nucleus and binds to hypoxia‒response elements (HREs) in the promoter of IL22R1, where it is upregulated. Increased IL22R1 surface expression enhances responsiveness to IL-22, leading to activation of the STAT3 signaling pathway. This promotes the proliferation and survival of intestinal stem cells (ISCs), facilitating epithelial regeneration and ultimately protecting against radiation-induced intestinal damage

Article Snippet: Recombinant murine IL-22 protein (rmIL22) and CHIR99021 were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Functional Assay, Inhibition, Staining, Isolation, Cell Culture, Control, Activity Assay, Activation Assay

Journal: iScience

Article Title: IL-22 and IL-23 regulate the anticryptococcal response during Cryptococcus deuterogattii infection

doi: 10.1016/j.isci.2024.111054

Figure Lengend Snippet:

Article Snippet: Murine IL-22 Standard ABTS ELISA Development Kit , PeproTech© , Cat#900-K257.

Techniques: Purification, Blocking Assay, Staining, Recombinant, Enzyme-linked Immunosorbent Assay, Activation Assay, Knock-Out, Software